Journal: Microscopy and Microanalysis

Article Title: Assessment of Medicago Based Systems for the Production of Human Proteins: Microscopy Analysis of the Subcellular Deposition Patterns of the Recombinant Product

doi: 10.1017/s1431927612012718

Figure Lengend Snippet: Figure 2. Immunolocalization of L-PGDS by confocal microscopy in the leaf (a) and root (b) tissue of transgenic Medicago truncatula (projection of optical sections). Incubation with primary antibody anti-L-PGDS (ab61866, Abcam) and secondary antibody anti-rabbit conjugated with a red fluorochrome (Alexa Fluor 594, Invitrogen).

Article Snippet: Incubation with primary antibody anti-L-PGDS (ab61866, Abcam) and secondary antibody anti-rabbit conjugated with a red fluorochrome (Alexa Fluor 594, Invitrogen).

Techniques: Confocal Microscopy, Transgenic Assay, Incubation

Journal: Frontiers in Oncology

Article Title: Elucidating the molecular markers and biological pathways associated with extrahepatic cholangiocarcinoma: a transcriptome sequencing study

doi: 10.3389/fonc.2024.1417374

Figure Lengend Snippet: The top 20 up-regulated and down-regulated DEGs in GEO.

Article Snippet: After blocking with 3% hydrogen peroxide (Maxim Biotechnologies) for 30 minutes and 10% normal goat serum (Maxim Biotechnologies) in PBS for 1 hour, sections were incubated overnight at 4°C with a primary antibody against PTGDS (1:100, cusabio, CSB-PA018969HA01HU),ITIH2(1:100, SAB 31939),LSAMP(1:50, Sangon Biotech, D123848),HBB(1:50, cusabio,CSB-PA010150LA01HU),.

Techniques:

Journal: Frontiers in Oncology

Article Title: Elucidating the molecular markers and biological pathways associated with extrahepatic cholangiocarcinoma: a transcriptome sequencing study

doi: 10.3389/fonc.2024.1417374

Figure Lengend Snippet: The KEGG pathways analyses of 22 hub genes.

Article Snippet: After blocking with 3% hydrogen peroxide (Maxim Biotechnologies) for 30 minutes and 10% normal goat serum (Maxim Biotechnologies) in PBS for 1 hour, sections were incubated overnight at 4°C with a primary antibody against PTGDS (1:100, cusabio, CSB-PA018969HA01HU),ITIH2(1:100, SAB 31939),LSAMP(1:50, Sangon Biotech, D123848),HBB(1:50, cusabio,CSB-PA010150LA01HU),.

Techniques: Activity Assay

Journal: eLife

Article Title: Oligodendrocyte-lineage cell exocytosis and L-type prostaglandin D synthase promote oligodendrocyte development and myelination

doi: 10.7554/eLife.77441

Figure Lengend Snippet:

Article Snippet: L-PGDS overexpressing transgenic mouse strain ( Ptgds -TG, line B7) was originally from Urade, cryopreserved by JCRB Laboratory Animal Resource Bank ( ).

Techniques: Recombinant, Purification, Software, Sequencing, Staining, Membrane

Journal: Aging (Albany NY)

Article Title: Transferrin is responsible for mediating the effects of iron ions on the regulation of anterior pharynx-defective-1α/β and Presenilin 1 expression via PGE 2 and PGD 2 at the early stage of Alzheimer’s Disease

doi: 10.18632/aging.101615

Figure Lengend Snippet: The key role of COX-2 in regulating the expression of APH-1α/1β and PS1 during the course of AD progression. ( A ) Three-month-old COX-2 Tg mice were injected (i.c.v) with NS398 (1 μg/5 μl) for 48 h (n=12). ( B ) In select experiments, n2a cells were transfected with COX-2 cDNA constructs in the absence or presence of NS398 treatment (10 μM). ( C ) In separate experiments, C57BL/6 mice were injected (i.c.v) with Aβ (500 ng/5 μl) in the absence or presence of NS398 (1 μg/5 μl) for 48 h. ( D ) In distinct experiments, n2a cells were treated with Aβ oligomers (1 μM) in the absence or presence of NS398 (10 μM) for 48 h. The mRNA and protein levels of APH-1α/1β and PS1 were determined by qRT-PCR and western blots, respectively. GAPDH and β-actin served as internal controls. The data represent the means±S.E. of all the experiments. * p<0.05 ; ** p<0.01 compared with C57BL/6, vector-transfected or vehicle-treated n2a controls. # p<0.05 with respect to COX-2 Tg mice or Aβ-treated mice alone.

Article Snippet: COX-2 or L-PGDS cDNA plasmids were obtained from Origene Technologies (Rockville, MD, USA), and subcloned to the pCMV6-XL vector.

Techniques: Expressing, Injection, Transfection, Construct, Quantitative RT-PCR, Western Blot, Plasmid Preparation

Journal: Aging (Albany NY)

Article Title: Transferrin is responsible for mediating the effects of iron ions on the regulation of anterior pharynx-defective-1α/β and Presenilin 1 expression via PGE 2 and PGD 2 at the early stage of Alzheimer’s Disease

doi: 10.18632/aging.101615

Figure Lengend Snippet: PGE 2 and PGD 2 antagonistically regulated the expression of APH-1α/1β and PS1 in n2a cells. ( A, C ) n2a cells were treated with Fe (10 μM) in the absence or presence of siRNA-targeted mPGES-1 or L-PGDS cDNA. ( B, D ) n2a cells were treated with PGE 2 (10 μM) or PGD 2 (1 μM) for 48 h. APH-1α/1β and PS1 were determined by qRT-PCR and western blots, respectively. GAPDH and β-actin served as internal controls. * p<0.05 ; **p<0.01 compared with vehicle-treated controls. # p<0.05 with respect to Fe-treatment alone.

Article Snippet: COX-2 or L-PGDS cDNA plasmids were obtained from Origene Technologies (Rockville, MD, USA), and subcloned to the pCMV6-XL vector.

Techniques: Expressing, Quantitative RT-PCR, Western Blot